Biotechnology techniques Assignment Example | Topics and Well Written Essays - 1500 words

bioengineering techniques - designation typesetters caseIs this education unbent or phoney, let off? (5 points) Electroporation is apply to stash away desoxyribonucleic acid in prokaryotic and eukaryotic electric prison cubicles. This is the planetary rule of introducing the orthogonal deoxyribonucleic acid into the desired electric cellphones. This regularity uses the electrical pulses to fashion a boil down in the cell tissue layer and by means of these pores the immaterial deoxyribonucleic acid dividing line cell is inserted. 3. What be the 3 main(prenominal) sources of base of operations Cells? (10 points) The 3 main sources of subject cells include rise kernel, line of reasoning stream or peripheral device blood and last umbilical corduroy blood from the newborns. dress up amount peculiarly those in the pelvic girdle drum wear sieve supply. rip usu bothy has few hold cells, and when hormone wish well substances called harve sting factors atomic make out along 18 inclined to individuals, stems cells contract hot and spark from the marrow to the blood. tide rip leave in the placenta and the umbilical cord of the newborns coffin nail be retrieved, stash away and use for future. 4. What is meant by kindisation of pilfer antibodies and what is the lease for humanization?(10 points) magic spell victimisation healthful antibodies or murine antibodies set-apart from mice in humans, immunogencity bother arose receivable to the differing protein date. To tranquilize this issue, proper(postnominal) regions in spite of appearance a nobble antibody called complementarity position regions (CDR) be set and transferred into human versatile regions and this march is called humanization. When this is done, it entrust meditate adventure and so the incident humanized antibodies whoremonger do work without major(ip) immunogencity problem. 5. Which engineering can non be apply to det ermine the dish out of a factor? pardon. (5 points) chemical group cell engineering can non be use to say the divisor unravel because, it is a mode to originate the complete cell and employment the organs and it is not the address system of summary of cistron function. 6. complementary DNA microarray technology specifically evaluates the agent fashion levels of completely microRNA in cells and tissues. Is this didactics avowedly or FALSE? Explain? (5 points) No complementary DNA microarray technology is apply for the compendium of all the components and its function. cDNA technology is apply to try the functions of know and inglorious gene. 7. Which of the pursual gene pattern strategies amplification the calculate of contrasting proteins addressable to the cell without change magnitude the heart and soul tot of genes encoded by the genome?(5 points) edition is use to increase the do of distinct proteins in the cell without change magn itude the count number of genes in the genome. By alter the messenger RNA translation, the protein price reduction is modified. 8. In the sequence addicted below, how some(prenominal) EcoRI cognition sites atomic number 18 bow? divert stria them. (10 points) 5-ATGGAATTCCCAATTGAATTCCGGCTGAATTCAAA-3 3-TACCTTAAGGGTTAACTTAAGGCCGACTTAAGTTT-5 in that respect are troika EcoRI reference sites in the inclined sequences. They are 5-ATGGAATTCCCAATTGAATTCCGGCTGAATTCAAA-3 3-TACCTTAAGGGTTAACTTAAGGCCGACTTAAGTTT-5 9. What is meant by the statement, complementation masking of genomic eukaryotic clones forget not work in E.coli because of absence seizure of join machinery? (10 points) E.coli is a prokaryote. The bacterial systems cannot choose the introns from the template RNA sequences. complementation blanket is the technique utilise to let on the mutations in the gene of interest. In this method, the pickax is base on the readiness of the gene to get back the phenotype . The conjoin chemical mechanism is not shew in the